Tools of Recombinant DNA Technology: Restriction Enzyme & DNA Fragments

Recombinant DNA technology, also known as genetic engineering or gene splicing, is a set of molecular techniques that involve the manipulation of DNA to create new combinations of genes, typically from different organisms. The goal is to generate recombinant DNA molecules that carry desired traits or functions. This technology has a wide range of applications in medicine, agriculture, and industry.

Restriction Enzymes

• Each restriction enzyme recognizes a speci…

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Tools of Recombinant DNA Technology: Cloning Vector and Gene Transfer

Parts of Cloning Vector

• The ori is a specific DNA sequence from which replication starts. When a piece of DNA is linked to this sequence, it can replicate within the host cells. The ori also controls the copy number of the linked DNA. Vectors with origins that support high copy numbers are desirable for obtaining multiple copies of the target DNA.
• A selectable marker is a gene that confers resistance to a particular antibiotic or other selective agent. In addi…

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