Fidelity of Replication: Replication Errors Involving the Strand Misalignments, Accessory Proteins Contribution to Fidelity, Multiprotein Replication Complexes Fidelity, DNA Repair Fidelity

Replication Errors Involving the Strand Misalignments

• During DNA synthesis in vitro, every DNA polymerase whose fidelity has been studied produces deletion or addition mistakes.
• The rates of deletion and addition errors can be as high as or higher than base substitution rates.
• The generation of addition and deletion errors has been explained by a number of models using mismatched DNA strands.

Template-Primer Slippage

• Slippage of the template and primer strands results in misma…

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TRNA Identity

Introduction

• Transfer RNA, also known as tRNA, is an adaptor molecule made of RNA that, in eukaryotes, typically has a length of 76 to 90 nucleotides.
• The link between genetic information encoded in DNA as well as its expression in the proteins is provided by transfer RNAs.
• Three years after Crick formulated the “adapter theory, ” tRNAs were found.
• This sparked interest in the development and tRNA recognition mechanisms of the aminoacyl-tRNA synthetases, the enzymes that acti…

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